Bunton Instrument Company, Inc.

DIGITAL IMAGING

Digital photomicroscopy has totally replaced silver halide photomicroscopy. Kodachrome film is now discontinued, slide projectors have been replaced by DLP and power point presentations. Image archiving, printing and display is SOP these days.

Key decisions must be made by the microscopist prior to making a final selection of digital components such as a camera, PC operating system, displayer, motorized microscope components and software. CMOS or CCD sensor in the camera? PC or Mac? Motorized focus or stage x/y travel? Simple image capture or quantification of the image (image analysis)? What can I afford today and where do I want to be with my digital imaging system a year from now?

CCD cameras have an edge over CMOS when it comes to light sensitivity. Peltier cooled CCD cameras for fluorescence microscopy are rather typical these days. These cameras eliminate dark current noise from low light emissions. The sensors are usually made by Sony or Kodak. The Bunton CCD cameras use Sony sensors and are connected to Windows PCs or Mac via USB2.

Here is a CCD Bunton camera mounted on an older Leitz microscope with a c-mount zoom lens.

Proper set up and use of the microscope is the first step to successful digital photomicroscopy. Even (Koehler) illumination, cleanliness of optical surfaces/samples and finding the best combination of resolution and contrast are critical. GIGO.

ZOOM MACROSCOPE XSZ - (1:6.5 ZOOM RANGE)


MZ MACROZOOM WITH EXTRA FEATURES

The new MZ (6:1) macrozoom is the next step up from the XSZ macrozoom. This unit (as shown in the photo) offers an iris diaphragm to control depth of focus and contrast. There is also a version with detents (click-stops) on the zoom for calibration and measurement accuracy. Add a digital camera and connect directly to your PC or purchase our newest XGA-HD15 color camera for direct high resolution imaging on any computer display or projector ... without having to connect to the PC!

The MZ is shown with a very nice X/Y mechanical stage that includes a theta rotation table for small sample manipulations. The fiber optic illuminator is connected to a mini-fiber optic ringlight for high intensity, shadow-free illumination of 3D objects. A basic incident light stand offers high stability and a very reasonable pricetag. Contact us for special optical/camera package prices that include the MZ, Infiniprobe and low power macro lens with our new XGA-HD15 camera included.


   

RETROFLUOR 2 INCIDENT LIGHT FLUORESCENCE MICROSCOPY SYSTEM WITH DIGITAL PELTIER-COOLED CAMERA

This is a great alternative to very expensive stereo microscopes and photomacroscopes from the microscope makers. This unit is primarily made in America and features our own vertical illuminator and cube hosting. The filter cubes come from Chroma in Vermont and the optical microscope if from Infinity Photo Optical in Boulder, CO. RetroFluor 2 is easily mounted to support stands to meet your exact needs and lighting can be metal halide, LED or high intensity halogen.

The unit accepts one filter module only but they are easily interchanged with any filter set in Chroma arsenal. The GFP set is the most popular for live organism studies. The photo shows the Bunton RetroFluor 2 mounted on a support stand we have made for industrial samples. It is ideal for small animal fluorescence studies as well.

A Peltier-cooled small format is the ideal choice but with the dual port bean-splitter (as shown) you can also connect your DSLR camera at the same time….or two small format cameras as required (i.e. mono and color etc). Shown with a Volpi flexible fiber optic light guide and high intensity LED light source. The optical front end accepts a variety of KC long working distance “macro” optics or regular infinity corrected microscope objective…including water immersion objectives for intravital studies.

Basic Retro Fluor 2 instruments start at about $5,000.00 with a blank filter module included. Filters, support stand, camera and illumination are not included.

CURRENT DIGITAL CAMERA LINEUP FROM BUNTON INSTRUMENT…

HDMI 1080p CAMERAS (COLOR CMOS):
#0771080A SD card and on/off crossline $1,100.00
#0771080C SD card and 8-line generator $1,200.00
#075TCHD DUAL PROCESSOR to use with or without PC connected(no SD card) $1,250.00
#0779280 SD card with software (no PC)and on-screen interactive live measurement functions using standard mouse. $1,750.00
COLOR CCD CAMERAS (use PC or MAC):
#075TCH5 5MP ICX282AQ $1,500.00
#0781400C 1.4MP ICX285AQ-Cooled $2,600.00
MONOCHROME CCD CAMERAS (use PC or MAC):
#0781400M 1.4MP ICX285AK-COOLED $2,600.00
#075674M 2.8MP ICX674ALG 2/3” $3,750.00
#0781400M 2.8MP ICX674ALG-COOLED SINGLE STAGE PELTIER $4,250.00
#075674IIM 2.8MP ICX674ALG-COOLED DUAL STAGE PELTIER $5,375.00

SMART- CAM HDMI 1080P

COOLED CCD
5MP COLOR CCD

All CCD cameras include basic image processing software that will run in Windows or Mac operating systems. We can also offer the correct camera couplers to your particular microscope. Contact Jim Averill (micro3d@comcast.net) with any questions or details about your exact requirements. We are always happy to send you sample images we have captured using these cameras. Send us your specimen and we will capture images and return your sample without charge.

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12610 Jesse Smith Road · Mt. Airy, Maryland 21771
Phone: 301-831-3434
· Fax: 301-831-3424 · E-Mail: micro3d@comcast.net

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